ABSTRACT
Nos últimos anos vários produtos cosméticos contendo alfahidroxiácidos (AHAs) têm sido desenvolvidos e estão disponíveis no comércio, causando uma grande revolução em relação ao tratamento da pele fotoenvelhecida. Com o aumento da produção desses produtos, torna-se necessário o desenvolvimento e validação de novas metodologias analíticas para determinar os AHAs contidos em preparações cosméticas, tanto para o controle da qualidade do produto como para a segurança do consumidor. Nesta pesquisa os AHAs tartárico, glicólico, lático e mandélico foram analisados simultaneamente por eletroforese capilar (CE) e cromatografia líquida de alta eficiência (CLAE). OS métodos foram validados avaliando-se os parâmetros de linearidade, limite de quantificação, precisão e exatidão...
In recent years many new cosmetic products containing alpha-hydroxy acids (AHAs) have been developed and are commercially available, causing a great revolution to the treatment of the photoaging. With the increase of the production of these products, there is a need of development and validation of analytical methods for quantitative determination of AHAs, both for quality control purposes and to avoid excessive concentrations that could cause consumers undesirable side-effects. In this research, AHAs like tartaric, glycolic, lactic and mandelic acids were analyzed simultaneously using capillary electrophoresis (CE) and high performance liquid chromatography (HPLC). The methods were validated evaluating the parameters of linearity, limit of quantitation, precision and accuracy. The separation and determination for tartaric, glycolic and lactic acids using CE involved an uncoated silica capillary with 75 µm i.d. and total length of 57 cm, 50 cm to the detector, with a solution containing 10 mmol.L-1 potassium phthalate as the background electrolyte and 0.5 mmol.L-1 cetyltrimethylammonium bromide as the electroosmotic flow modifier, pH 4.1, the applied voltage was -20 kV with indirect detection at 254 nm, the operating temperature was 35°C. The separation and determination for tartaric, glycolic and lactic acids using HPLC involved an Sinergy Hydro RP-18 Phenomenex...
Subject(s)
Consumer Product Safety , Cosmetics , Skin Aging , Chromatography, Liquid/methods , Electrophoresis, Capillary , Quality ControlABSTRACT
The aim of this research was to determine the sun protection factor (SPF) of sunscreens emulsions containing chemical and physical sunscreens by ultraviolet spectrophotometry. Ten different commercially available samples of sunscreen emulsions of various manufactures were evaluated. The SPF labeled values were in the range of 8 to 30. The SPF values of the 30 por cento of the analyzed samples are in close agreement with the labeled SPF, 30 por cento presented SPF values above the labeled amount and 40 por cento presented SPF values under the labeled amount. The proposed spectrophotometric method is simple and rapid for the in vitro determination of SPF values of sunscreens emulsions